Our Health Library information does not replace the advice of a doctor. Please be advised that this information is made available to assist our patients to learn more about their health. Our providers may not see and/or treat all topics found herein. The serum protein electrophoresis (SPEP) test measures specific proteins in the blood to help identify some diseases. Proteins are substances made up of smaller building blocks called amino acids. Proteins carry a positive or a negative electrical charge, and they move in fluid when placed in an electrical field. Serum protein electrophoresis uses an electrical field to separate the proteins in the blood serum into groups of similar size, shape, and charge. Blood serum contains two major protein groups: albumin and globulin. Both albumin and globulin carry substances through the bloodstream. Using protein electrophoresis, these two groups can be separated into five smaller groups (fractions): Each of these five protein groups moves at a different rate in an electrical field and together form a specific pattern. This pattern helps identify some diseases. Serum protein electrophoresis is most often done to help diagnose and monitor a wide variety of conditions. These include: In general, there's nothing you have to do before this test, unless your doctor tells you to. A health professional uses a needle to take a blood sample, usually from the arm. When a blood sample is taken, you may feel nothing at all from the needle. Or you might feel a quick sting or pinch. There is very little chance of having a problem from this test. When a blood sample is taken, a small bruise may form at the site. Test results for each protein group are given as a percentage of the total amount of serum protein. To obtain the actual amount of each fraction, a test that measures the total serum protein must also be done. Each lab has a different range for what's normal. Your lab report should show the range that your lab uses for each test. The normal range is just a guide. Your doctor will also look at your results based on your age, health, and other factors. A value that isn't in the normal range may still be normal for you. High values may be caused by many conditions. Some of the most common are shown here. Low values may be caused by many conditions. Some of the most common are shown here. Current as of: May 13, 2023 Author: Healthwise Staff Current as of: May 13, 2023 Author: Healthwise Staff Clinical Review Board This information does not replace the advice of a doctor. Healthwise, Incorporated disclaims any warranty or liability for your use of this information. Your use of this information means that you agree to the Terms of Use and Privacy Policy. Learn how we develop our content. To learn more about Healthwise, visit Healthwise.org. © 1995-2024 Healthwise, Incorporated. Healthwise, Healthwise for every health decision, and the Healthwise logo are trademarks of Healthwise, Incorporated.Serum Protein Electrophoresis (SPEP) Test
Test Overview
Why It Is Done
How To Prepare
How It Is Done
How It Feels
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Results
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Low values
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All Healthwise education is reviewed by a team that includes physicians, nurses, advanced practitioners, registered dieticians, and other healthcare professionals.
All Healthwise education is reviewed by a team that includes physicians, nurses, advanced practitioners, registered dieticians, and other healthcare professionals.
Our Health Library information does not replace the advice of a doctor. Please be advised that this information is made available to assist our patients to learn more about their health. Our providers may not see and/or treat all topics found herein. Current as of: May 13, 2023 Author: Healthwise Staff Clinical Review BoardSerum Protein Electrophoresis (SPEP) Test
All Healthwise education is reviewed by a team that includes physicians, nurses, advanced practitioners, registered dieticians, and other healthcare professionals.